Project Details
Description
Epithelial-mesenchymal transition (EMT) is a characteristic of cancer cell intravasation and metastasis. Moreover, recent studies have demonstrated the intimate involvement of EMT in the development of metastatic prostate cancer. In light of the role of EMT in tumor progression and metastasis, EMT regulators have been proposed as a predictive marker of response to cancer treatment.
The AMPK has been demonstrated with ability to inhibit motility of cancer cells and downregulated in advanced prostate cancer. Therefore, the therapeutic potential of pharmacological modulation of the AMPK represent a promising therapeutic strategy for advanced prostate cancer. Berberine is a Chinese herb
with the ability to active of AMPK and has been demonstrated effects of anti-metastasis in some cancer cell lines. But, the inhibitory effect of berberine on invasive behavior and EMT of prostate cancer cells remain largely unknown. In this study, we demonstrated that berberine in suppressing cancer cell invasiveness was illustrated by its dosedependent inhibitory effect on the igration and invasion of highly aggressive PC-3 cells. To understand the mechanism of berberine-regulated EMT,we perform EMT-PCR array analysis. We found that berberine-mediated suppression phenotype of EMT through inhibited EMT-regulators expression, especially inhibition of Nodal, AHNAK nucleoprotein,
Fibroblast Growth Factor Binding Protein 1 (FGFBP1), desumoylating isopeptidase 1 (PPPDE2) and TGF 1 expression. Moreover, we also found the expression of Nodal and PPPDE2 were significantly upregulated in the metastatic prostate cancer tissues compared to the primary prostate cancer tissues (p<0.05 and p<0.001, respectively). Taken together, these results reveal that cell metastatic abilities could be inhibited by berberine through the suppression of Nodal and PPPDE2 expression in human prostate cancer cells.
The AMPK has been demonstrated with ability to inhibit motility of cancer cells and downregulated in advanced prostate cancer. Therefore, the therapeutic potential of pharmacological modulation of the AMPK represent a promising therapeutic strategy for advanced prostate cancer. Berberine is a Chinese herb
with the ability to active of AMPK and has been demonstrated effects of anti-metastasis in some cancer cell lines. But, the inhibitory effect of berberine on invasive behavior and EMT of prostate cancer cells remain largely unknown. In this study, we demonstrated that berberine in suppressing cancer cell invasiveness was illustrated by its dosedependent inhibitory effect on the igration and invasion of highly aggressive PC-3 cells. To understand the mechanism of berberine-regulated EMT,we perform EMT-PCR array analysis. We found that berberine-mediated suppression phenotype of EMT through inhibited EMT-regulators expression, especially inhibition of Nodal, AHNAK nucleoprotein,
Fibroblast Growth Factor Binding Protein 1 (FGFBP1), desumoylating isopeptidase 1 (PPPDE2) and TGF 1 expression. Moreover, we also found the expression of Nodal and PPPDE2 were significantly upregulated in the metastatic prostate cancer tissues compared to the primary prostate cancer tissues (p<0.05 and p<0.001, respectively). Taken together, these results reveal that cell metastatic abilities could be inhibited by berberine through the suppression of Nodal and PPPDE2 expression in human prostate cancer cells.
| Status | Finished |
|---|---|
| Effective start/end date | 1/1/13 → 10/31/13 |
Keywords
- Prostate Cancer
- Epithelial-mesenchymal transition
- AMP-activited Protein Kinase
Fingerprint
Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.