Investigation on the Role of Cytoskeleton Reorientation and Calcium Ion Transportation in Static Magnetic Field Induced Mesenchymal Stem Cells Proliferation

Stem cell is an important resource in regenerative medicine. However, how to obtain a sufficient number of stem cells for cell therapy is still an study issue in clinical applications. Static magnetic field (SMF) was reported to enhance the proliferation of several cell types. Whether or not SMF also has a positive effect on mesenchymal stem cells (MSCs) proliferation is still unclear. Therefore, the aim of this study is to investigate the possible effect of SMFs on MSC proliferation and/or differentiation. In addition, the possible action mechanism between SMF and alternation of intracellular molecules also will be detected. For methodology, a mesenchymal stem cell line (WJMSC) will be used in this study proposal. The cells will be cultured with various SMFs up to 0.4-Tesla. Mechanical structure and status of the lipid bilayer of the SMF exposed cells will be examined using fluorescence polarization-depolarization assay. The intracellular calcium ions of the SMF-exposed cells will be analyzed using Fura-2AM labeling. The cytoskeletons of SMF exposed and unexposed control cells will be microscopically observed by labeling with actin fluorescence. To discern the possible signaling cascade involved when in the proliferation/differentiation effect of the SMF on the WJMSC cells, cell viability will be checked when the tested cells are cultured with inhibitors of ERK, JNK and p38, respectively. Real time polymerase chain reaction will be used to detect the activation level of p38 signal cascade when the stem cells are exposed to SMFs. After finish all the experiments, we will provide more insight into the mechanism of SMF exposure of hMSCs. The results of this study will be a useful guide and reference for future advanced investigations and applications of regenerative medicine.