Investigation on the Effects of Smf on the Promotion of Neuritogenesis and Inhibition of Neurodegeneraton

Neural disease can be roughly divided into two catalogues, i.e., traumatic injury and neurodegenerative disease. For traumatic injury, regeneration of neural cells has become the most important issue. However, its practice in clinical is still limited. Inhibitions of inflammatory response of neural cells and reduction of proinflammatory cytokine release from microglia cells are two major strategies for prevention of neurodegenerative disease. In our previous studies, we found that static magnetic fields (SMF) can promote osteoblastic differentiation. This is because SMFs change the orientations of lipid molecular on the cellular membrane resulting in a reduction of binding capability between endotoxin (LPS) and its receptor (TLR). That is, SMF stimulation has capability to reduce the excess inflammatory response in cellular level. In addition, after a series of tests, we found such effects were cell-type independent. According to these findings, our rationale of this proposal is that SMFs also have neurogeneration and anti-neuroinflammation effects. Accordingly, in this proposal, we try to design experiments to test this hypothesis. In the first year of this proposal, we will test the hypothesis that SMF can promote nitrite outgrowth or not. In the second year, we will establish in vitro experiments using microglia cells to test the anti-neuroinflammation effects of SMF. In the third year, animal model will be carried out to test the hypothesis in vivo. After finish all the three-year tests, we will provide more insight into the mechanism of SMF stimulation, and the results of this study will be a useful guide and reference for future clinical practice.