Immune-Modulation of Nk Cells in Kidney Disease: Enhancing Therapeutic Performance by Crispr Gene Editing( I )

In this proposal, we aim to investigate the modulation of NK cells using CRISPR technology. Specifically, we will focus on the roles of immune-modulation regulators in NK-92 cells and isolated NK cells. The study will involve characterizing the transcriptome profiles, activation and exhaustion markers, cytokine profiles, cytotoxic activity, and exosome profiles of CRISPR-edited NK cells. Through co-culture experiments with injured kidney cells and kidney organoids, we will evaluate precise gene-editing strategies to optimize NK cell functions for kidney injury repair. Moreover, CRISPR-edited NK cell-derived exosomes have the potential to serve as novel therapeutic agents for immune-mediated kidney diseases. To the best of our knowledge, this is the first study to explore the application of CRISPR technology for modulating NK cells in the context of kidney injury repair.