Genetic and Epigenetic Regulation of Superoxide Dismutase and Interleukin-8 in Periodontal Disease Activities and Treatment Effectiveness

Periodontal disease is an inflammatory disease. It has been shown that single nucleotide polymorphisms (SNPs) and DNA methylation may contribute to genetic susceptibility to inflammatory and redox regulation in an individual with periodontitis. Our preliminary study explored ten salivary inflammatory and redox biomarkers and primary results showed that there were significant positive correlations between salivary manganese superoxide dismutase (MnSOD) or interleukin 8 (IL-8), and periodontal disease activities (r=0.17-0.23, N=186). The genotype of MnSOD (T47C, rs4880) and IL-8 (A251T, rs4073) were both associated with periodontal pocket depth restoration (N=96). This project will be conducted in the next two years following MOST (Ministry of Science and Technology) project of 2016 and anticipated to reach 300 periodontal disease subjects (ICD9: 523). The aim of this project is continually to explore the association between MnSOD and IL-8 of DNA methylation as well as genetic polymorphisms in disease activities and treatment effectiveness of periodontal disease. In the first year, the project will determine whether genetic polymorphism influence the levels of MnSOD and IL-8 as well as periodontal disease activities and treatment effectiveness. In the second year, the project will evaluate the effects of MnSOD or IL-8 DNA methylation on periodontal disease activities and treatment effectiveness. After the Research Ethics Committee of the Taipei Medical University Joint Institutional Review Board (Taipei, Taiwan) approve this project, patients with periodontal disease (ICD9: 523) are recruited. All participants provide written informed consent prior to the questionnaire interview and specimen collection. Salivary MnSOD and IL-8 levels are determined by Magnetic Bead Panel kit. DNA extraction from oral swab is used for genetic polymorphism and that from subgingival calculus during root planning is used for methylation analysis. Genetic Polymorphism is determined by using real-time PCR. After bisulfite conversion, DNA methylation is determined by using methylation specific real-time PCR. This research will clarify the important roles of periodontal treatment in the relationship among phenotypic, genetic and epigenetic alterations in MnSOD and IL-8. We anticipate the integrated and consecutive project providing invaluable findings to enhance the quality and effectiveness of periodontal treatment.