Confounding Factors Associated with Immature Testicular Graft Survival for Fertility Preservation - Transgenic Mouse Model

This research grant spans a multidisciplinary innovation experiment involving reproductive preservation, biomolecular imaging and regenerative medicine. The purpose is to explore the most frequent multiple factors to affect the survival rate of immature testicular grafts which deserves to improve the survival rate of the immature graft from lacking spermatogenesis to spermatogenesis over time in the long run. Our long term series of applied bioluminescence imaging in vivo to track the grafts under 3-D in vivo imaging system (IVIS) system to analyze the graft survival in a longitudinal real-time model that may create much faster and more convicing data on a quality and quantity basis even in the experiment to adjust the drugs delivery or able to reduce multiple error trials. In the experiment, the same animals were used for continuous observation to reduce untoward sacrified mouse model that may meet the 3R principles of substitution, reduction and sophistication. While the fate mapping of immature testicular graft remains in infance. We intend explore different types of anti-rejection drugs for the seminiferious tubules may expose to host blood to generate anti-sperm antobodies over time by host growing age with initiating spermatogenesis. The other issue should be considered the regenerated capacity of different immature age. The third aspect is the ischemic reperfusion injury during the acute and early period. The anti-oxidant drugs have been widley studied for the reperfusion injury for a while. These series studies are based all long on in vivo and in vitro evidence to track the subsequent development of immature transplanted tissue to strengthen the effects of different drug treatments at different ages on the preservation of immature male gonadal tissue. Further evidence still need tissue biopsy and blood biochemical analysis to build a better way for regeneration among the immature tissue. Fertility preservation of sexual immature male cancer patients is a prospective and promising study which may undergo imminent chemotherapy or radiotherapy injury to loss reproductive function. Young male victims are unable to spermatogenesis that frozen immature graft is the solution to preserve the fertility, followed by spermatogonial stem cells transplantation or immature graft in vivo. With the advent of the best choice for the sexual immature children facing cancer treatment is subject to the under development of combined frozen graft in a more comprehensive in vivo model.