Research output per year
Research output per year
Description
Accession Number: GSE106664
Platform:
GPL15523: Phalanx Mouse OneArray Ver 2
Organism: Mus musculus
Published on 2017-11-09
Summary:
The cell identities of CD49f+GSCs were further identified by comparing them with the E11.5 PGCs and P2 GSCs. The transcriptomic analysis revealed that the CD49f+GSCs had 1/3 similar genes profile to the E11.5 PGCs and P2 GSCs. Further gene ontology (GO) analysis demonstrated that the E11.5 PGCs, P2 GSCs, and CD49f+GSCs shared the partial similar gene expression profile of pluripotency regulation signaling pathway, PI3K-AKT signaling, chemokine signaling, and HIF-1 signaling.
Overall Design:
Isolation of the gonadal cells from 67 E11.5 ICR embryos, and testicular cells from 80 newborn to P2 ICR mice (0- to 2-day-old postpartum), followed by stem cell colony formation under 5% O2 hypoxia for 7 days, and subjected to transcriptome analysis. Meanwhile, CD49f+GSCs from 100 newborn to P2 ICR mice were purified and subjected to transcriptome analysis.
Contact:
Name: Yung-Che Kuo
Organization: Taipei Medical University
Address: 250 Wuxing Taipei 11031 Taiwan
Email: [email protected]
Name: Sean Chang
Organization: PhalanxBiotech Group
Deparment: R&D
Address: 6F, NO.6 Technology Road 5 Hsinchu 30078 Taiwan
Email: [email protected]
Phone: 886-3-5781168
Platform:
GPL15523: Phalanx Mouse OneArray Ver 2
Organism: Mus musculus
Published on 2017-11-09
Summary:
The cell identities of CD49f+GSCs were further identified by comparing them with the E11.5 PGCs and P2 GSCs. The transcriptomic analysis revealed that the CD49f+GSCs had 1/3 similar genes profile to the E11.5 PGCs and P2 GSCs. Further gene ontology (GO) analysis demonstrated that the E11.5 PGCs, P2 GSCs, and CD49f+GSCs shared the partial similar gene expression profile of pluripotency regulation signaling pathway, PI3K-AKT signaling, chemokine signaling, and HIF-1 signaling.
Overall Design:
Isolation of the gonadal cells from 67 E11.5 ICR embryos, and testicular cells from 80 newborn to P2 ICR mice (0- to 2-day-old postpartum), followed by stem cell colony formation under 5% O2 hypoxia for 7 days, and subjected to transcriptome analysis. Meanwhile, CD49f+GSCs from 100 newborn to P2 ICR mice were purified and subjected to transcriptome analysis.
Contact:
Name: Yung-Che Kuo
Organization: Taipei Medical University
Address: 250 Wuxing Taipei 11031 Taiwan
Email: [email protected]
Name: Sean Chang
Organization: PhalanxBiotech Group
Deparment: R&D
Address: 6F, NO.6 Technology Road 5 Hsinchu 30078 Taiwan
Email: [email protected]
Phone: 886-3-5781168
| Date made available | 2017 |
|---|---|
| Publisher | Gene Expression Omnibus |
Research output
- 1 Article
-
IGF-1R Promotes Symmetric Self-Renewal and Migration of Alkaline Phosphatase+ Germ Stem Cells through HIF-2α-OCT4/CXCR4 Loop under Hypoxia
Kuo, Y. C., Au, H. K., Hsu, J. L., Wang, H. F., Lee, C. J., Peng, S. W., Lai, S. C., Wu, Y. C., Ho, H. N. & Huang, Y. H., Feb 13 2018, In: Stem Cell Reports. 10, 2, p. 524-537 14 p.Research output: Contribution to journal › Article › peer-review
Open Access30 Citations (Scopus)