Additional file 1: Fig. S1. Working schemes for immunological analysis of nanoparticle IT-101 in mice. Fig. S2. Significant changes after 16â h IT-101 treatment were presented by dot plots. Fig. S3. Cytokine assay. Fig. S4. Changes of cytokine expression after CPT treatment. Fig. S5. Significant changes after 216â h treatment were presented by dot plots. Fig. S6. Early stage of tumorigenesis in F1B transgenic mice.