Additional file 8: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

Figure S8. HDAC1 stronger associated with the E-cadherin promoter in Hop62 cells overexpressing Ubc9 compare with the vector control cells. The normal mouse IgG and HDAC1 antibodies were used to pull down protein-DNA complexes in Hop62 cells with or without Ubc9 overexpression; and the E-cadherin promoter level in the samples was determined by PCR using a gene-specific primer set. Input, an aliquot of each sample was prepared and used as a template for PCR to examine the level of the E-cadherin promoter before immunoprecipitation (IP). (PDF 25 kb)