YAP1 overexpression (OE) leads to increased stemness in colon cancer cells. (A) A significantly increased CD133+ cell percentage was found in the YAP1 OE cells as compared to their wild-type counterparts as demonstrated by our flow cytometric analysis. (B) YAP1-overexpressing HCT116 and DLD-1 cells were found to form a significantly higher number of tumor spheres (*P ≤ 0.05, **P ≤ 0.01). (C) Comparative western blots of wild-type and YAP1 overexpressing HCT116 and DLD-1 cells. An increased expression of β-catenin, NF-kB, vimentin, and Kras is associated with YAP1 overexpression. (PPTX 396 kb)
Date made available2017

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