Additional file 1 of Humanized bispecific antibody (mPEG × HER2) rapidly confers PEGylated nanoparticles tumor specificity for multimodality imaging in breast cancer

  • En Shuo Liu (Contributor)
  • Chih Kuang Wang (Contributor)
  • Kuo-Hsiang Chuang (Contributor)
  • Fang Ming Chen (Creator)
  • Tung Ho Wu (Contributor)
  • Chiao Yun Chen (Creator)
  • I. Ju Chen (Creator)
  • Rui An Lin (Creator)
  • Y. M. Wang (Contributor)
  • Yun-Chi Lu (Contributor)
  • Tzu Yi Liao (Contributor)
  • Huei Jen Chen (Creator)
  • Tian Lu Cheng (Creator)
  • Yi-An Cheng (Creator)



Additional file 1: Table S1. The BsAb-conjugation rate of αHER2/PEG-NPs. Table S2. The Characterization of BsAb/mPEG-NPs. Figure S1. Immunogenicity of humanized BsAbs. We cocultured dendritic cells differentiated from human PBMCs with autologous CD4+ T cells and stimulated with control medium (represented as DC+T), PHA (as positive control), PEG×HER2, PEG×DNS, respectively, for 5 days. Then, we detected the proliferation of CD4+ T cells by ATPlite assay. Bars, SD. CPM, counts per minute; PBMC, peripheral blood mononuclear cell; PHA, phytohemagglutinin.
Date made available2020

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